Process Development Co-Op (PhD)
- Opportunity Profile:
- Project Description:Acetate metabolic engineering project consists of two phases. Phase 1 is the proof-of-concept study on model strain K12, which is ongoing; Phase 2 is the application of dynamic metabolons technology in industrial production strains. The incumbent performs experiments at Devens facility of BMS. Optimized constructs and induction conditions obtained from Phase 1 study on model strain will be applied to the production strain to modulate acetate metabolism, but recombinant protein titer will be added as a critical parameter to further optimize induction conditions and timing. The metabolic profiles and production performance will be compared side by side between the engineered and non-engineered production strains. Bench-scale bioreactors/fermenters and the associated scale-down models may be needed for comparative performance evaluations.Year of Individual(s) Requested: PhDMajor(s): Chemical Engineering or BiotechnologyLocation of Assignment(s): Devens, MADuration of Assignment(s): January 2020 to June 2020 (Co-Op)Required skills:Genetic manipulationConstruction of protein expression vectorCreation of E. coli protein expression strainsSDS-PAGEBioreactor/fermenter operation and data interpretationMetabolite measurement and microbial metabolism data interpretation
The incumbent will learn and perform metabolic engineering to improve bacterial strains for recombinant protein productions under industrial setting.